100nm NHS-Activated Silver Nanoparticle Conjugation Kit (3 Reactions)

100nm NHS-activated Silver Nanoparticle Conjugation Kit have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to silver nanoparticles.

The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the silver nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester silver nanoparticles supplied with the kit.

This mini kit contains 3 individual reaction vials with lyophilized NHS-activated silver nanoparticles for performing three independent conjugation reactions.

Kit Components

  • 100nm NHS-Activated Silver Nanoparticles (lyophilized) – 3 vials
  • Protein Resuspension Buffer
  • Reaction Buffer
  • Quencher Solution

Features & Benefits

  • Results in covalently bound ligand and more stable conjugate.
  • Fast and convenient one-step conjugation reaction with no pre-activation requirements.
  • Spacer between the silver nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
  • Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

Gold Nanoparticle Specifications

Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between silver surface and NHS-group)

Core diameter: 100nm

Concentration: OD=18 (when dissolved to a final volume of 100ul)

Particles per ml: ~7.2E+10 (when dissolved to a final volume of 100ul)

Absorbance (lambda max): 480-520nm

100nm silver nanoparticles UV-VIS spectrum

Diameter Peak SPR Wavelength (nm) NPS/ml* Wt. Conc. (mg/ml)* Size Dispersity (+/-nm) Particle Volume (nm3) Surface Area (nm2) Surface/Volume Ratio Particle Mass (g) Molar Mass (g/mol) Molar Concentration*
100 480-520 3.6E+09 2.00E-02 <10% 5.24E+05 3.14E+04 0.06 5.49E-15 3.31E+09 5.98E-12

* All concentrations as indicated at an optical density of 0.92 (OD=0.92, i.e. absorbance at peak SPR wavelength with a 1cm pathlength)