Multiplex Nanoparticle Detection Kit for Western Blot (Red and Blue)

Composition: 1 ml of affinity purified anti-rabbit IgG secondary antibody conjugated nanoparticles (blue colour) and 1 ml of anti-mouse IgG secondary antibody conjugated nanoparticles (red colour).
Antibodies raised in: Goat
Detection type: Colorimetric
Working dilution: 1:50
Supplied in: 20mM Tris (pH 8.0), 150mM NaCl, 20% glycerol (v/v), 1% BSA
Volume: 1 ml of each nanoparticle probe – Enough material to probe 3-5 standard size (80 mm x 70 mm) blotted PVDF or nitrocellulose membranes.

Cytodiagnostics unique multiplex nanoparticle colorimetric secondary detection kit allows for simultaneous detection of up to two antigens in immunoblotting applications such as western blots and dot-blots.

Detection can be performed visually and does not require the use of expensive imaging equipment, which is required for multiplex western blots with fluorescent detection.

Multiplex dot blot
Figure 1. Simultaneous detection of two different antigens using our multiplex nanoparticle detection kit in a vertical flow dot blot assay in less than 5 minutes.

Features & Benefits
  • Direct visual detection – No expensive imaging equipment required
  • Probing of two antigens simultaneously offers significant time savings
  • Provides a permanent signal
  • Good sensitivity (ng) for most targets
Applications
  • Western Blot
  • Dot-Blot
  • Lateral Flow
  • Vertical Flow