Required reagents not included in kit
- Primary antibody/antibodies - For antigen capture on the membrane and conjugation to gold nanoparticles (detection probe).
Assay Principle
To detect an antigen using this toolkit a primary antibody is spotted onto the nitrocellulose membrane encapsulated within the specially designed plastic test cartridge.
When the sample is applied to the cartridge any antigen present will bind to the capture antibody immobilized on the membrane. This binding event is subsequently detected by the formation of a sandwich through the binding of a Gold NanoUrchin conjugated primary antibody to the bound antigen. A positive sample will be indicated by the formation of a blue spot on the membrane, figure 1.
Figure 1. Illustration of the appearance of the Miriad™ cartridge after applying a gold conjugate (conjugated to an antibody against the antigen of interest) when target is present (left) and not present in the sample (right).
