Ni-NTA Gold Nanoparticles: Precisely Engineered for Optimal Protein Binding
Our Ni-NTA gold nanoparticles are crafted with meticulously engineered surface chemistry, featuring a PEG spacer terminated with a nitrilotriacetic acid (NTA) group that is chelated to a Ni2+ ion. These nanoparticles are ideal for specific applications in binding proteins with a C-, or N-terminal 6X His tag.
- Composition: Ni-NTA gold nanoparticles
- Core Size: Available in 5nm, 10nm, 15nm, up to 100nm
- Absorption max: 515-520nm
- Optical Density (OD): 50
- Particles per ml: 2.73E+15
- Ni-NTA Surface Density: Approximately 1/nm²
- Formulation: Supplied in USP grade purified water
- Pack Sizes: Available in sizes ranging from 5nm to 100nm
Properties:
Ni-NTA functionalized gold nanoparticles offer an efficient solution for rapid generation of gold conjugates of any histidine-tagged protein. These conjugates can be effectively used as probes in various downstream applications. Our gold nanoparticles are more than 95% spherical, ensuring uniformity with a size distribution (CV <12%). Available in 12 distinct sizes from 5nm to 100nm, these nanoparticles undergo rigorous quality control using dynamic light scattering to ensure size and monodispersity, with detailed lot specifications provided for each batch.
Storage Instructions:
Store the product at 4°C, away from direct sunlight. Do NOT freeze. When stored as specified, the product remains stable for at least twelve months.
Applications:
These Ni-NTA gold nanoparticles are particularly suitable for binding and conjugation processes involving 6X His-tagged proteins. Their precise engineering makes them an invaluable tool for researchers needing reliable and reproducible results in protein-related studies.
