20nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)

Cytodiagnostics 20nm NHS-activated Gold Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to gold nanoparticles.

The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester gold nanoparticles supplied in the kit.
Kit Components
  • 20nm NHS-Activated Gold Nanoparticles (lyophilized)
  • Protein Resuspension Buffer
  • Reaction Buffer
  • Quencher Solution
Features & Benefits
  • Results in covalently bound ligand and more stable conjugate.
  • Fast and convenient one-step conjugation reaction with no pre-activation requirements.
  • Spacer between the gold nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
  • Precisely engineering surface coating that reduces non-specific protein binding in biological assays.
Gold Nanoparticle Specifications
Surface functionality: NHS-ester (~ 1 NHS group/nm2) (5kDa PEG-spacer between gold surface and NHS-group)
Core diameter: 20nm
Concentration: OD=20 (when dissolved to a final volume of 1ml)
Particles per ml: ~1.3E+13 (when dissolved to a final volume of 1ml)
Absorbance (lambda max): 524nm