20nm NHS-Activated Silver Nanoparticle Conjugation Kit (MIDI Scale-Up Kit)

20nm NHS-activated Silver Nanoparticle Conjugation Kit have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to silver nanoparticles.

The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the silver nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester silver nanoparticles supplied with the kit.

This MIDI kit contains 1 vial with lyophilized NHS-activated silver nanoparticles for performing one independent scale-up conjugation reaction.

Kit Components

• 20nm NHS-Activated Silver Nanoparticles (lyophilized) – 1 vial
• Protein Resuspension Buffer
• Reaction Buffer
• Quencher Solution

Features & Benefits

• Results in covalently bound ligand and more stable conjugate.
• Fast and convenient one-step conjugation reaction with no pre-activation requirements.
• Spacer between the silver nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
• Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

Gold Nanoparticle Specifications

Surface functionality: NHS-ester (~ 1 NHS group/nm2) (5kDa PEG-spacer between silver surface and NHS-group)

Core diameter: 20nm

Concentration: OD=50 (when dissolved to a final volume of 1ml)

Particles per ml: ~9.2E+12 (when dissolved to a final volume of 1ml)

Absorbance (lambda max): 395-410nm

* All concentrations as indicated at an optical density of 2.5 (OD=2.5, i.e. absorbance at peak SPR wavelength with a 1cm pathlength)