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Product Overview

The Rabbit IgG ELISA Kit is a precise and sensitive assay designed for the quantitative measurement of Rabbit IgG levels in serum, plasma, and cell culture supernatants.

This assay operates on the antibody sandwich principle. The microtiter plate is coated with a capture antibody specific to the Rabbit IgG Fc region and has been pre-blocked and stabilized to form the solid phase. Samples, standards, and controls are directly added to the wells. After unbound IgG is removed through washing, an HRP-conjugated Detection Antibody Solution is added, binding to the heavy and light chains of the captured Rabbit IgG. Following additional washes, tetramethylbenzidine (TMB) Substrate Solution is introduced, resulting in a blue color proportional to the bound Rabbit IgG. The reaction is stopped using Stop Solution, turning the blue color to yellow. The optical density (OD) of the yellow product is measured at 450 nm using a microtiter plate reader.

Key Benefits:

• Ease of Use: Ready-to-use format; simply add standards and samples directly to the wells without blocking or rehydration steps.
• High Performance: Offers high sensitivity, specificity, and reproducibility.
• Validated Applications: Fully validated for use with serum, plasma, and cell culture supernatants.
• Flexible Design: Includes a 96-well plate that can be broken into 12 x 8 well strips for flexible assay setup.

Download: ELISA Product Brochure

Download: ELISA Service Brochure

 Product Sheet – Rabbit IgG ELISA Kit

 Handbook – Rabbit IgG ELISA Kit

 Safety Data Sheet (SDS) – Rabbit IgG ELISA Kit

 Rabbit IgG Fc Lateral Flow Dipstick Assay Kit – Product Sheet

 ELISA Products Brochure

 ELISA Service Brochure

Product Data

* All concentrations as indicated at an optical density of 1 (OD=1, i.e. absorbance at peak SPR wavelength with a 1cm pathlength)

 Product Sheet

 Safety Data Sheet (SDS)

QC Conjugation Kit – Product Sheet

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