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Product Overview

The Human IgG ELISA Kit is a sensitive and precise assay designed for the quantitative determination of human IgG levels produced in vivo and for evaluating immunoglobulin secretion by hybridomas in vitro.

This assay uses the antibody sandwich principle. A microtiter plate, pre-coated with a capture antibody specific to the human IgG Fc region, has been blocked and stabilized to serve as the solid phase. Samples, standards, and controls are directly added to the wells. After unbound IgG is removed through washing, an HRP-conjugated Detection Antibody Solution is introduced, binding to the heavy and light chains of the captured human IgG. Following additional washes, a tetramethylbenzidine (TMB) Substrate Solution is added, producing a blue color proportional to the amount of bound IgG. The reaction is stopped using Stop Solution, turning the blue product to yellow, and the optical density (OD) is measured at 450 nm with a microtiter plate reader.

Key Benefits:

• Ease of Use: Ready-to-use format; standards and samples can be added directly to the wells with no need for blocking or rehydration.
• High Performance: Ensures high sensitivity, specificity, and reproducibility.
• Validated Applications: Fully validated for assessing human IgG in various sample types.
• Flexible Format: Includes a 96-well plate that can be broken into 12 x 8 well strips for customizable assay setup.

Download: ELISA Product Brochure

Download: ELISA Service Brochure

Product Specification

 Product Sheet – Human IgG ELISA Kit

 Handbook – Human IgG ELISA Kit

 Safety Data Sheet (SDS) – Human IgG ELISA Kit

 Human IgG Fc Lateral Flow Dipstick Assay Kit – Product Sheet

 ELISA Products Brochure

 ELISA Service Brochure

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