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Human TNF-alpha ELISA Kit
RN-CY-EL-007
Tumor Necrosis Factor alpha (TNF alpha) is a versatile proinflammatory cytokine belonging to the TNF superfamily. Its biologically active form is a homotrimer with a molecular mass of 52 kDa, while the monomeric form remains inactive. TNF alpha is produced as a transmembrane protein in humans and assembled intracellularly into a noncovalently linked homotrimer before being expressed on the cell surface. It is predominantly secreted by macrophages and monocytes during acute inflammation and plays a critical role in cellular signaling pathways that result in necrosis or apoptosis. TNF alpha is also essential for immune defense against infections and cancers.
ELISA, ELISA kit
Product Overview
Tumor Necrosis Factor alpha (TNF alpha) is a versatile proinflammatory cytokine belonging to the TNF superfamily. Its biologically active form is a homotrimer with a molecular mass of 52 kDa, while the monomeric form remains inactive. TNF alpha is produced as a transmembrane protein in humans and assembled intracellularly into a noncovalently linked homotrimer before being expressed on the cell surface. It is predominantly secreted by macrophages and monocytes during acute inflammation and plays a critical role in cellular signaling pathways that result in necrosis or apoptosis. TNF alpha is also essential for immune defense against infections and cancers.
The effects of TNF alpha are mediated through binding to TNFR-1 or TNFR-2. Both receptors exhibit similar affinity for TNF alpha and can activate NF-κB. However, TNFR-1 uniquely contains a cytoplasmic death domain that triggers apoptosis. Soluble forms of both receptors are present in human serum and urine, neutralizing TNF alpha’s biological activity.
Physiologically, TNF alpha is crucial for regulating immune responses. However, its overproduction or dysregulation can contribute to various diseases, including asthma, Crohn’s disease, rheumatoid arthritis, neuropathic pain, obesity, type 2 diabetes, septic shock, autoimmunity, and cancer. Its central role in chronic inflammatory and autoimmune diseases highlights its importance in both research and clinical applications.
ELISA Kit Benefits
- Ease of Use: Ready-to-use format, requiring only the addition of standards and samples directly into wells without extensive blocking or rehydration steps.
- Enhanced Performance: Delivers high sensitivity, specificity, and reproducibility.
- Reliable Validation: Fully validated for a range of sample types.
- Customizable Plate Design: 96-well plate is breakable into 12 x 8 well strips for flexible use.
This kit is an excellent tool for researchers in India conducting studies on chronic inflammatory and autoimmune diseases.
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Product Specification
| Protein name | TNF alpha |
| Alternate names |
Tumor necrosis factor alpha, TNF-α, APC1 protein, cachectin, TNFA, TNF-alphacachectin, TNFSF1A |
| Species reactivity | Human |
| Assay format | Solid-phase Sandwich ELISA (quantitative) |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Sample volume | 100 μL |
| Assay length | 4.5 hrs |
| Analytical sensitivity | <11 pg/mL |
| Assay range |
31.2 – 2000 pg/mL |
| Intra-assay CV | <2% |
| Inter-assay CV | <7% |
| Recovery (%) |
Serum: 97.7 |
| Detection & Instrument | Colorimetric, Microplate Reader |
| UniProt ID | (Human) Q9UBM5 |
Product Sheet – Human TNF-alpha ELISA Kit
Handbook – Human TNF-alpha ELISA Kit
Safety Data Sheet (SDS) – Human TNF-alpha ELISA Kit
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