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Human VEGF ELISA Kit
RN-CY-EL-004
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a 45 kDa homodimeric, disulfide-linked glycoprotein produced by various cell types that plays a key role in angiogenesis and vasculogenesis.
The VEGF family consists of VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E (Orf-VEGF), and placental growth factor (PIGF). Among the multiple isoforms of VEGF-A produced via alternative mRNA splicing from the VEGFA gene, VEGF165 is the most abundant and essential for angiogenesis.
ELISA, ELISA kit
Product Overview
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a 45 kDa homodimeric, disulfide-linked glycoprotein produced by various cell types that plays a key role in angiogenesis and vasculogenesis.
The VEGF family consists of VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E (Orf-VEGF), and placental growth factor (PIGF). Among the multiple isoforms of VEGF-A produced via alternative mRNA splicing from the VEGFA gene, VEGF165 is the most abundant and essential for angiogenesis.
VEGF governs several biological processes, including the proliferation and survival of endothelial cells, vascular permeability, macrophage cell migration, wound healing, bone formation, and the development of embryonic vasculature. VEGF-A interacts with receptors VEGFR-1 and VEGFR-2 to regulate angiogenesis and vascular permeability, while VEGF-C and VEGF-D primarily bind VEGFR-3 to control lymphangiogenesis. VEGF expression is stimulated by hypoxia and cytokines such as IL-1, IL-6, IL-8, and TNF-alpha.
ELISA Kit Benefits
- Ease of Use: Ready-to-use format; simply add standards and samples directly into the wells, eliminating the need for time-intensive blocking or rehydration steps.
- High Performance: Delivers high sensitivity, specificity, and reproducibility.
- Validated Reliability: Fully validated across a variety of sample types.
- Flexible Plate Design: 96-well plate is breakable into 12 x 8 well strips for customizable use.
This ELISA kit is a reliable and efficient solution for researchers in India studying angiogenesis, lymphangiogenesis, and related processes.
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Product Specification
| Protein name | VEGF |
| Alternate names | Vascular endothelial growth factor A, vascular endothelial growth factor A121, vascular endothelial growth factor A165, Vascular permeability factor, VEGF-A, VPF |
| Species reactivity | Human |
| Assay format | Solid-phase Sandwich ELISA (quantitative) |
| Sample type | Serum, Cell culture supernatant |
| Sample volume | 100 μL |
| Assay length | 4.5 hrs |
| Analytical sensitivity | <30 pg/mL |
| Assay range | 31.25 – 2000 pg/mL |
| Intra-assay CV | <8% |
| Inter-assay CV | <10% |
| Serum recovery | 95.3% |
| Detection & Instrument | Colorimetric, Microplate Reader |
| UniProt ID | (Human) P15692 |
Product Sheet – Human VEGF ELISA Kit
Handbook – Human VEGF ELISA Kit
Safety Data Sheet (SDS) – Human VEGF ELISA Kit
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