NanoDiagnostics: Advanced Materials for Innovative Solutions

Home

Navigation

Category

Nanoparticles (298) Lateral Flow (47) ELISA (18) Antibodies (39)

Materials

Gold Silver NanoUrchins Iron Oxide Quantom Dots Nano-Rods Assays Materials Immunoglobulin Biomarkers Reagents Consumables Custom Assay Development

Format

Spherical Conjugates Kits

Property

Standard Reactant Free Stabilized Endotoxin Free Organic Flow Cytometry Standards Custom Conjugation Protein/Antibody Passive Protein Adsorption NHS-Activated Maleimide-Activated OligoREADY Biotin AptamerREADY Amine Ni-NTA Azide Alkyne Methoxy Carboxyl DBCO Organic Soluble Water Soluble Passive Adsorption

Click Here to Find Customized Products

SON Filter
Application
Application
Application
Reset

Reset

ELISA Stop Solution

RN-CY- EL-C8-50ML

Product overview

The ELISA Stop Solution is a ready-to-use liquid acidic solution suitable to stop TMB substrate- peroxidase reaction. This product is suitable for all endpoint ELISAs using a TMB substrate for colorimetric analysis. TMB substrates react with peroxidase, yielding a soluble blue reaction product. When the TMB-peroxidase reaction is stopped using Stop Solution, the chromogen changes from blue to yellow, and hence the absorbance from 620 nm to 450 nm.

ELISA, ELISA kit

Storage Condition

Can be stored for up to 3 months at 2-8 °C. For precautionary handling, refer to the safety data sheet.

Application

  • Equilibrate the ELISA Stop Solution to room temperature (25°C) prior to use.
  • After the TMB substrate reaction until the desired intensity, add 50–100 µL stop solution to plate well.
  • Read absorbance at 450 nm within 10 minutes. For best results, sample absorbance should be monitored and stopped before values exceed 2.0 absorbance units.

 

 Product Sheet

 Safety Data Sheet (SDS)

Get in Touch

Contact Us

Related Products


ELISA Coating Buffer
✴ ELISA
ELISA kit

ELISA Detection Diluent
✴ ELISA
ELISA kit

Human VEGF ELISA Kit
✴ ELISA
ELISA kit

Gold NanoUrchins

Standard Gold NanoUrchins

Unique spiky nanoparticles for enhanced surface area and reactivity.

Functionalized NanoUrchins

Carboxylated, methylated, and biotin-functionalized options for specific applications.

Endotoxin-Free NanoUrchins

Ultra-clean nanoparticles for sensitive biological experiments and in vivo studies.

Gold Nanorods

Amine Gold Nanorods

Positively charged nanorods for electrostatic interactions and conjugations.

Carboxyl Gold Nanorods

Negatively charged nanorods for diverse chemical modifications.

Methyl Gold Nanorods

Hydrophobic nanorods for specialized applications in non-polar environments.

Silver Nanoparticles

Standard Silver Nanoparticles

High-quality silver nanoparticles for various research applications.

Reactant Free Silver Nanoparticles

Ultra-pure silver nanoparticles for sensitive experiments.

Endotoxin Free Silver Nanoparticles

Clean silver nanoparticles for biological and medical research.

Magnetic Nanoparticles

Organic Soluble Iron Oxide Nanoparticles

Specialized magnetic nanoparticles for use in organic solvents and non-polar environments.

Water Soluble Iron Oxide Nanoparticles

Hydrophilic magnetic nanoparticles for aqueous applications and biomedical research.

Applications

Magnetic separation, targeted drug delivery, MRI contrast agents, and more.

Quantum Dots

Water-Soluble Quantum Dots

Highly luminescent nanocrystals for aqueous applications and biomedical imaging.

Organic-Soluble Quantum Dots

Specialized quantum dots for use in organic solvents and optoelectronic devices.

Quantum Dots Kits

Complete sets for easy implementation in various research and development projects.