NanoDiagnostics: Advanced Materials for Innovative Solutions

Storage Condition

Can be stored for up to 1 month at 2-8 °C. Protect from moisture and light. No special shipping conditions or precautions are required.

Application

• Dilute the detection primary antibody to the desired concentration and volume in 1x ELISA Detection Diluent, mix well. Typically, detection antibody concentration can range from 0.05 – 0.5 µg/mL
• Add 100 µL of the diluted detection primary antibody solution in each well and incubate according to the ELISA protocol
• After incubation remove solution and wash the plate 3-4 times with 300 µL of (1x) ELISA Wash Buffer (EL-C2) and decant properly
• Dilute the secondary antibody-HRP or streptavidin-HRP conjugate (if biotinylated detection primary antibody was used) to the desired concentration and volume in 1x ELISA Detection Diluent.
• Add 100 µL of the diluted HRP conjugate solution in each well and incubate according to the ELISA protocol
• After incubation remove solution and wash the plate 4-5 times with 300 µL of (1x) ELISA Wash Buffer (EL-C2) and decant properly to prepare the plate for HRP-substrate addition.

 Product Sheet

 Safety Data Sheet (SDS)

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