NanoDiagnostics: Advanced Materials for Innovative Solutions

Kit Components

• 40nm Maleimide-Activated Gold Nanoparticles (lyophilized) – 3 vials
• Protein Resuspension Buffer
• Reaction Buffer
• Quencher Solution

Features & Benefits

• Results in covalently bound ligand and more stable conjugate.
• Fast and convenient one-step conjugation reaction with no pre-activation requirements.
• Spacer between the gold nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
• Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

Gold Nanoparticle Specifications

Surface functionality: Maleimide (~0.5 maleimide groups/nm2) (10kDa PEG-spacer between gold surface and maleimide-group)

Core diameter: 40nm

Concentration: OD=20 (when dissolved to a final volume of 100ul)

Particles per ml: ~1.4E+12 (when dissolved to a final volume of 100ul)

Absorbance (lambda max): 530nm

* All concentrations as indicated at an optical density of 1 (OD=1, i.e. absorbance at peak SPR wavelength with a 1cm pathlength)

 Product Sheet

 Safety Data Sheet (SDS)

QC Conjugation Kit – Product Sheet