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Gold NanoUrchin Nanoparticle Conjugation Optimization Kit – 50nm

RN-CY-GNO-50-100

Supplied with Kit

  • 50nm standard GoldNanourchins (100ml, conc. OD1)
  • 12 conjugation optimization buffers (1.5ml each)
  • 10% NaCl (100ml)
  • Protein resuspension buffer
  • Detailed conjugation optimization protocol

Lateral Flow, Nanoparticle

Kit contains enough material to optimize 10 protein conjugates, or one optimization reaction and scale-up production of 20ml of conjugate at a final optical density of OD3.

Gold NanoUrchin conjugates have been used for a wide range of biological applications including the use as probes in light and electron microscopy (TEM) and for membrane blotting (immunogold dot-blot); especially where large surface plasmon resonance shifts are required.

Stable Gold NanoUrchin conjugates can readily be prepared by passive adsorption of a protein to the Gold NanoUrchin surface, which is mediated by electrostatic and hydrophobic interactions between the protein and the surface layer of the colloidal gold.

Our Gold NanoUrchin protein conjugation optimization kits are supplied as complete and easy to use systems for increased success rates in the development of good quality Gold NanoUrchin conjugates.

50nm Gold NanoUrchins UV-VIS Spectrum
Diameter Peak SPR Wavelength (nm) NPS/ml* Wt. Conc. (mg/ml)* Molar Ext. (M-1cm-1) Size Dispersity (+/-nm) Particle Volume (nm3) Surface Area (nm2) Surface/Volume Ratio Particle Mass (g) Molar Mass (g/mol) Molar Concentration*
50 560 3.512E+10 4.45E-02 1.72E+10 <12% 6.54E+04 7.85E+03 0.12 1.27E-15 7.64E+08 5.83E-11

* All concentrations as indicated at an optical density of 1 (OD=1, i.e. absorbance at peak SPR wavelength with a 1cm pathlength)

 Product Sheet – Gold NanoUrchins

 Safety Data Sheet (SDS) – Gold NanoUrchins

 Gold NanoUrchins Brochure

 QC Conjugation Kit – Product Sheet

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