Streptavidin covalently conjugated gold nanoparticles can be used for secondary detection of biotinylated probes in assays such as lateral and vertical flow assays, immunoblotting, ELISA, light microscopy and electron microscopy applications.
When compared to traditional conjugates utilizing passive adsorption, covalent conjugation provides:
- Improved stability of the final conjugate
- Higher sensitivity in assays
- Reduced background and non-specific binding events
- Improved control over the final loading of protein onto the gold’s surface
Streptavidin gold conjugates can be used for convenient and fast conjugation of any biotinylated ligand such as antibodies and oligonucleotides to the gold surface.
Product Sheet And SDS
Gold nanoparticle core size: 5nm, 10nm, 15nm, 20nm,.........100nm.
Absorbance (lambda max.): 515-520nm
Optical density: Available at OD=3 and OD=10, select in product options
Conjugated protein: Streptavidin, from Streptomyces avidinii
Working dilution: 1:5-1:100
Supplied in: 1XPBS (pH 7.4), 20% glycerol (v/v), 1% BSA
Applications
Gold conjugates are suitable for use in immunoblotting, light microscopy, and electron microscopy applications.
Advantages of Gold Conjugates
- Sensitive technique (100pg or less in dot blotting when used together with our silver enhancement kit)
- This small gold nanoparticle conjugate is ideal for increasing sensitivity in immunohistochemistry protocols when used together with our silver enhancement kit for microscopy.
- Provides a permanent label
Storage/Stability
- Store undiluted in storage buffer at 2-8 degree Celsius. Stable for 4 months if stored as specified.
- Storage of conjugate at working dilution may result in performance loss.