Maleimide-Activated Gold Nanoparticles (MIDI Scale-up Kits)
Maleimide-Activated Gold Nanoparticle Conjugation Kit is expertly designed for the efficient, one-step conjugation of thiol-containing ligands to gold nanoparticles. This gold nanoparticle conjugation kit simplifies the process by eliminating the need for pre-activation or additional manipulation, which often results in suboptimal conjugates. The procedure is straightforward—just mix your protein with the pre-activated maleimide gold nanoparticles included in the kit for a seamless, high-yield reaction.
Each Maleimide-Activated Gold Nanoparticle Conjugation Kit is available with pre-made, ready-to-use mixtures in individual reaction vials, offering either 3 or 10 lyophilized maleimide-activated gold nanoparticles for three independent conjugation reactions.
Available Pack Sizes: 5nm, 10nm, 15nm, up to 100nm.
Kit Components:
Maleimide-Activated Gold Nanoparticles (lyophilized) - 3 vials or 10 vials
- Protein Resuspension Buffer
- Reaction Buffer
- Quencher Solution
Features & Benefits:
- Achieves covalently bound ligands, ensuring more stable gold nanoparticle conjugates.
- Fast and convenient one-step conjugation without the need for pre-activation.
- The spacer between the gold nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, preventing poor performance—common in conjugates prepared by passive adsorption.
- Precisely engineered surface coating reduces non-specific protein binding in biological assays.
Gold Nanoparticle Specifications:
- Surface functionality: Maleimide (~0.5 maleimide groups/nm²) (5kDa PEG-spacer between gold surface and maleimide-group)
- Core diameter: Available in 5nm, 10nm, 15nm, up to 100nm
- Concentration: OD=20 (when dissolved to a final volume of 100μl)
- Particles per ml: ~1.1E+14 (when dissolved to a final volume of 100μl)
- Absorbance (lambda max): 515-520nm