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Spherical Conjugates KitsProperty
Standard Reactant Free Stabilized Endotoxin Free Organic Flow Cytometry Standards Custom Conjugation Protein/Antibody Passive Protein Adsorption NHS-Activated Maleimide-Activated OligoREADY Biotin AptamerREADY Amine Ni-NTA Azide Alkyne Methoxy Carboxyl DBCO Organic Soluble Water Soluble Passive Adsorption
80nm AptamerREADY Gold NanoUrchin Conjugation Kit (10 reactions)
RN-CY-AUC-80-2
Cytodiagnostics AptamerREADY™ Gold NanoUrchin Conjugation Kits have been optimized for high efficiency one-step conjugation of thiolated aptamers directly to the gold surface.
The kit contains ready-to-use pre-made mixtures. No activation, manipulation or time consuming “salt-aging” steps are required for conjugation. Simply mix your thiol-modified aptamer with the pre-activated gold NanoUrchins supplied in the kit. Conjugation of the aptamer is achieved by the formation of a strong and stable gold-thiol bond.
Lateral Flow, AptamerREADYGold NanoUrchins
Kit Components
- 10 vials of 80nm AptamerREADY™ Gold NanoUrchins (lyophilized) – Sufficient for generation of 10 x 0.2 ml gold conjugate at OD=10
Features & Benefits
- Results in thiol-aptamer bound directly to the gold surface through a stable thiol-gold bond.
- Fast and convenient one-step conjugation reaction with no pre-activation requirements.
- Does not require time consuming salt aging procedures saving you valuable research time.
Gold Nanoparticle Specifications
Gold Surface: Proprietary AptamerREADY™-coating
Core diameter: 80nm
Concentration: OD=2 (when dissolved a final volume of 1 ml)
Particles per ml: ~1.56E+10 (when dissolved to a final volume of 1 ml)
Absorbance (lambda max): 620nm
| Diameter | Peak SPR Wavelength (nm) | NPS/ml* | Wt. Conc. (mg/ml)* | Molar Ext. (M-1cm-1) | Size Dispersity (+/-nm) | Particle Volume (nm3) | Surface Area (nm2) | Surface/Volume Ratio | Particle Mass (g) | Molar Mass (g/mol) | Molar Concentration* |
| 80 | 620 | 7.82E+09 | 4.06E-02 | 7.70E+10 | <10% | 2.68E+05 | 2.01E+04 | 0.075 | 5.20E-15 | 3.13E+09 | 1.30E-11 |
* All concentrations as indicated at an optical density of 1 (OD=1, i.e. absorbance at peak SPR wavelength with a 1cm pathlength)
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