NanoDiagnostics: Advanced Materials for Innovative Solutions

Supplied with Kit

• 20nm standard gold nanoparticles (100ml, concentration OD1)
• 12 conjugation optimization buffers (1.5ml each)
• 10% NaCl solution (100ml)
• Protein resuspension buffer
• Detailed conjugation optimization protocol

This kit includes sufficient materials to optimize 10 protein conjugates or perform one optimization reaction and scale up production of 20ml of conjugate at a final optical density of OD3.

Note: This kit is intended for use exclusively with standard or reactant-free gold nanoparticles.

Gold nanoparticle conjugates have been extensively applied in various biological studies, such as probes in light and electron microscopy (TEM) and for membrane blotting techniques, including immunogold dot-blot.

Stable gold nanoparticle conjugates can be efficiently prepared through passive adsorption of a protein onto the nanoparticle surface. This process is mediated by electrostatic and hydrophobic interactions between the protein and the nanoparticle’s surface layer.

The protein conjugation optimization kits are provided as all-inclusive, easy-to-use systems to improve the success rates of developing high-quality gold nanoparticle conjugates.

Product Data

* All concentrations as indicated at an optical density of 1 (OD=1, i.e. absorbance at peak SPR wavelength with a 1cm pathlength)

 Product Sheet

 Safety Data Sheet (SDS)

QC Conjugation Kit – Product Sheet

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