NanoDiagnostics: Advanced Materials for Innovative Solutions

Supplied with Kit

• 40nm standard gold nanoparticles (100ml, concentration OD1)
• 12 conjugation optimization buffers (1.5ml each)
• 10% NaCl solution (100ml)
• Protein resuspension buffer
• Detailed conjugation optimization protocol

The kit includes sufficient materials to optimize 10 protein conjugates or to carry out one optimization reaction and scale up production of 20ml of conjugate with a final optical density of OD3.

Note: This kit is designed for use only with standard or reactant-free gold nanoparticles.

Gold nanoparticle conjugates are widely used in biological research, including as probes for light and electron microscopy (TEM) and for membrane blotting techniques such as immunogold dot-blot.

Stable gold nanoparticle conjugates can be efficiently created via passive adsorption of proteins onto the nanoparticle surface. This process relies on electrostatic and hydrophobic interactions between the protein and the surface layer of the colloidal gold.

The protein conjugation optimization kits are provided as complete, user-friendly systems that enhance the success rate of developing high-quality gold nanoparticle conjugates.

Product Data

* All concentrations as indicated at an optical density of 1 (OD=1, i.e. absorbance at peak SPR wavelength with a 1cm pathlength)

 Product Sheet

 Safety Data Sheet (SDS)

QC Conjugation Kit – Product Sheet

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