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80nm Maleimide-Activated Gold NanoUrchins Conjugation Kit (3 Reactions)
RN-CY-MUG10K-80-1
Cytodiagnostics 80nm Maleimide-activated Gold NanoUrchin Conjugation Kit has been optimized for high efficiency one-step conjugation of thiol-containing ligands to gold Gold NanoUrchins.
The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanourchins is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated Maleimide gold nanourchins supplied in the kit.
This mini kit contains 3 individual reaction vials with lyophilized maleimide-activated gold nanourchins for performing three independent conjugation reactions.
Lateral Flow, Maleimide-ActivatedGold NanoUrchins
Kit Components
- 80nm Maleimide-Activated Gold NanoUrchins (lyophilized) – 3 vials
- Protein Resuspension Buffer
- Reaction Buffer
- Quencher Solution
Features & Benefits
- Results in covalently bound ligand and more stable conjugate.
- Fast and convenient one-step conjugation reaction with no pre-activation requirements.
- Spacer between the gold surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
- Precisely engineering surface coating that reduces non-specific protein binding in biological assays.
Gold Nanoparticle Specifications
Surface functionality: Maleimide (~1 Maleimide group/nm2) (10kDa PEG-spacer between gold surface and Maleimide-group)
Core diameter: 80nm
Concentration: OD=20 (when dissolved to a final volume of 100ul)
Particles per ml: ~1.56E+11 (when dissolved to a final volume of 100ul)
Absorbance (lambda max): 620nm
| Diameter | Peak SPR Wavelength (nm) | NPS/ml* | Wt. Conc. (mg/ml)* | Molar Ext. (M-1cm-1) | Size Dispersity (+/-nm) | Particle Volume (nm3) | Surface Area (nm2) | Surface/Volume Ratio | Particle Mass (g) | Molar Mass (g/mol) | Molar Concentration* |
| 80 | 620 | 7.82E+09 | 4.06E-02 | 7.70E+10 | <10% | 2.68E+05 | 2.01E+04 | 0.075 | 5.20E-15 | 3.13E+09 | 1.30E-11 |
* All concentrations as indicated at an optical density of 1 (OD=1, i.e. absorbance at peak SPR wavelength with a 1cm pathlength)
QC Conjugation Kit – Product Sheet
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