Anti-6X His IgG is an antibody produced in mouse that binds specifically to a sequence of 6 repeating histidine residues, known as a His tag.
These His tags are typically expressed at either the C- or N-terminal regions of recombinant proteins and aid in purification and isolation using immobilized metal affinity chromatography. His tags have a strong affinity for Ni2+ through chelation.
Anti-6X His conjugated gold nanoparticles are suitable for use in applications such as lateral flow, immunoblotting, light microscopy, and electron microscopy.
Product Sheet And SDS
Gold nanoparticle core size: 5nm, 10nm, 15nm, 20nm,.......100nm.
Absorbance (lambda max.): 515-520nm
Optical density: Available at OD=3 and OD=10, select in product options
Conjugated protein: Mouse Anti-6X His Antibody
Working dilution: 1:1-1:100
Supplied in: 1XPBS (pH 7.4), 20% glycerol (v/v), 1% BSA
Applications
Sensitive probe for detection of recombinant proteins with a C-terminal, N-terminal, or internal 6X Histidine epitope.
Suitable for use in immunoblotting, lateral flow assays, light microscopy, and electron microscopy applications among others.
Storage/Stability
- Store undiluted in storage buffer at 2-8 degree Celsius. Stable for 4 months if stored as specified.
- Storage of conjugate at working dilution may result in performance loss.