Anti-biotin IgG is an antibody produced in goat that binds specifically to the biotin molecule.
Biotin is a small molecule that plays a large role in many processes for a wide range of organisms. Biotin is also extensively used in protein labelling due to its small size and relative stability when attached to a protein or antibody.
The anti-biotin antibody is an ideal choice for detection of biotinylated proteins as well as purification and isolation.
Anti-biotin conjugated gold nanoparticles are suitable for use in applications such as lateral flow, immunoblotting, light microscopy, and electron microscopy.
Product Sheet And SDS
Gold nanoparticle core size: 5nm, 10nm, 15nm, 20nm,.......100nm.
Absorbance (lambda max.): 515-520nm
Optical density (OD): Available at OD=3 and OD=10, select in product options
Conjugated antibody: Affinity purified goat anti-biotin antibody
Raised in: Goat
Clonality: Polyclonal
Isotype: IgG
Specificity: Biotin
Working dilution: 1:1-1:100 (application dependant)
Supplied in: 20mM Tris (pH 8.0), 150mM NaCl, 20% glycerol (v/v), 1% BSA
Applications
Anti-Biotin Gold conjugates are suitable for detection of biotinylated moieties in lateral flow, immunoblotting, light microscopy, and electron microscopy applications.
Advantages of Gold Conjugates
- Sensitive technique (down to 100pg or less in dot-blots when used together with our silver enhancement kit)
- Provides a permanent label