Conjugation Kit for Multiplex Western Blot Detection (Red and Blue)

Kit Components

Contains sufficient amount of material to generate two nanoparticle primary antibody probes (1 ml of each). When used as a mixture for multiplex colorimetric detection, these probes can be used to probe 3-5 standard (80 mm x 70 mm) PVDF or nitrocellulose membranes after blotting.

Reagents included:

  • NHS-Activated Gold Nanoparticles
  • NHS-Activated Gold NanoUrchins
  • Protein Resuspension Buffer
  • Reaction Buffer
  • Quencher Solution
  • Detailed Product Guide

Cytodiagnostics nanoparticle conjugation kit western blots allows for simultaneous colorimetric detection of up to two antigens (Figure 1.) when conjugated to two independent primary antibodies. Detection can be performed visually and does not require the use of expensive imaging equipment, which is required for multiplex western blots using fluorescent antibody probes.

multiplex dot blot
Figure 1. Simultaneous detection of two different antigens using conjugates made with our conjugation kit for multiplex nanoparticle detection kit in a vertical flow dot blot assay in less than 5 minutes.
This kit contains probes made from two different types of nanoparticles. Each probe provides a different color upon detection, i.e. blue and red. The two different colors are achieved by the unique optical properties of each nanoparticle probe and allows for direct visual multiplex colorimetric detection down into the lower nanogram range.

The kit contains ready-to-use materials, no manipulation or activation of the nanoparticles is required prior to conjugation. Simply mix your primary antibodies of interest with their respective nanoparticle probe and incubate for 1-2 hours to conjugate and your multiplex probes are ready to be used. A detailed product guide is included with the product.

Features & Benefits
  • Direct visual detection – No expensive equipment required
  • Probing of two antigens simultaneously offers significant time savings
  • Provides a permanent signal
  • Good sensitivity (ng) for most targets
When used to conjugate primary antibodies this product is suitable for colorimetric multiplex detection in applications such as:
  • Western Blot
  • Dot-Blot
  • Lateral Flow
  • Vertical Flow