Unique AptamerREADY™ gold conjugation kits have been optimized for high efficiency one-step conjugation of thiolated aptamers directly to the gold surface of particles with diameters up to 100nm.
The kit contains ready-to-use pre-made mixtures. No activation, manipulation, or time consuming "salt-aging” steps are require for conjugation. Simply mix your reduced thiol-modified aptamer with the pre-activated gold nanoparticles supplied with the kit. Conjugation of the aptamer is achieved by the formation of a strong and stable gold-thiol bond without any additional linkers.
Features & Benefits
- Allows conjugation of aptamers to gold nanoparticles with sizes between 5nm-100nm.
- Fast and convenient one-step conjugation reaction with no pre-activation requirements or manipulation of the gold nanoparticles.
- Does not require time-consuming "salt-aging” procedures.
- Results in a thiol-aptamer conjugated directly to the gold surface without any linkers.
Working with oligonucleotides? Check out our OligoREADY™ gold nanoparticle kits for easy and improved conjugation!
AptamerREADY Gold Nanoparticle Conjugation Kit (10 reactions)
We Are available with 5nm, 10nm, 15nm,......100nm. AptamerREADY Gold Nanoparticle Conjugation Kit (3 Reactions)
AptamerREADY™ Gold Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugation of thiolated aptamers directly to the gold surface.
The kit contains ready-to-use pre-made mixtures. No activation, manipulation or time consuming "salt-aging" steps are required for conjugation. Simply mix your thiol-modified aptamer with the pre-activated gold nanoparticles supplied in the kit. Conjugation of the aptamer is achieved by the formation of a strong and stable gold-thiol bond.
Kit Components
- 3 vials of 5nm AptamerREADY™ Gold Nanoparticles (lyophilized) - Sufficient for generation of 3 x 0.2 ml gold conjugate at OD=10
Features & Benefits
- Results in thiol-aptamer bound directly to the gold surface through a stable thiol-gold bond.
- Fast and convenient one-step conjugation reaction with no pre-activation requirements.
- Does not require time consuming salt aging procedures saving you valuable research time.
Gold Nanoparticle Specifications
- Gold Surface: Proprietary AptamerREADY™-coating
- Core diameter: 5nm
- Concentration: OD=2 (when dissolved a final volume of 1 ml)
- Particles per ml: ~1.09E+14 (when dissolved to a final volume of 1 ml)
- Absorbance (lambda max): 515-520nm