NHS-activated Silver Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to silver nanoparticles.
NHS-Activated Silver Nanoparticle Conjugation Kit(3 Reactions, 5Reactions)
NHS-activated Silver Nanoparticle Conjugation Kit have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to silver nanoparticles.
NHS-Activated Silver Nanoparticle Conjugation Kit (3 Reactions,10 Reactions)
The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the silver nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester silver nanoparticles supplied with the kit.
This mini kit contains 3 individual reaction vials with lyophilized NHS-activated silver nanoparticles for performing three independent conjugation reactions.
Kit Components
- NHS-Activated Silver Nanoparticles (lyophilized) - 3 vials, 5 vials
- Protein Resuspension Buffer
- Reaction Buffer
- Quencher Solution
Features & Benefits
- Results in covalently bound ligand and more stable conjugate.
- Fast and convenient one-step conjugation reaction with no pre-activation requirements.
- Spacer between the silver nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
- Precisely engineering surface coating that reduces non-specific protein binding in biological assays.
Gold Nanoparticle Specifications
- Surface functionality: NHS-ester (~ 1 NHS group/nm2) (5kDa PEG-spacer between silver surface and NHS-group)
Core diameter: 10nm, 20nm, 30nm,...100nm.
- Concentration: OD=50 (when dissolved to a final volume of 100ul)
- Particles per ml: ~7.2E+13 (when dissolved to a final volume of 100ul)
- Absorbance (lambda max): 390-405nm