Gold Nanoparticle Conjugation Optimization Kit, (Dot-blot)
Supplied in Kit:
- 20nm and 30nm standard gold nanoparticles (100ml each, conc. OD1)
- 12 conjugation optimization buffers (1.5ml each)
- 10% NaCl (100ml)
- Protein resuspension buffer
- Detailed conjugation optimization protocol
Kit contains enough material to optimize 10 protein conjugates, or one optimization reaction and scale-up production of 20ml of conjugate at a final optical density of OD3.
Gold nanoparticle conjugates have been used for a wide range of biological applications including the use as probes in light and electron microscopy (TEM) and for membrane blotting (immunogold dot-blot).
Stable gold nanoparticle conjugates can readily be prepared by passive adsorption of a protein to the gold nanoparticle surface, which is mediated by electrostatic and hydrophobic interactions between the protein and the surface layer of the colloidal gold.
Our gold nanoparticle protein conjugation optimization kits are supplied as complete and easy to use systems for increased success rates in the development of good quality gold nanoparticle conjugates.