Carboxyl
Carboxyl functionalized gold nanoparticles have precisely engineered surface chemistry that enables coupling to various moieties that react with carboxylic acids.
One of the most popular applications of this interaction is EDC/NHS coupling. The EDC activates the carboxylic acid so that it can react with NHS and acquire new functionality. The introduction of the NHS group then allows it to react with primary amines, which enables covalent coupling to proteins and antibodies with exposed amine-containing residues such as lysine.
This permanent linkage has thus provided a gold nanoparticle label to the molecule in question and allows easier monitoring and localization within cells.
Carboxyl gold nanoparticles are available with diameters ranging from 5nm – 100nm and are also available with 2 different spacer lengths (3 kDa and 5 kDa).
This provides the customer with the necessary tools fully optimize their experiment.
Ranges Available in 5nm, 10nm, 15nm,........100nm, in PEG 3000-SH And PEG5000-SH.
(carboxyl-PEG5000-SH) Gold Nanoparticles
- Composition: Carboxyl gold nanoparticles
- Core Size: 5nm,10nm,15nm,.......100nm
- Absorption max: 515-520nm
- Optical Density (OD): 50
- Particles per ml: 2.73E+15
- Carboxyl Surface Density: ~1/nm2
- Formulation: Supplied in USP grade purified water
Properties
PEGylated Gold Nanoparticles with carboxyl functional groups are made using a unique proprietary protocol resulting in particles with superior size distribution and shape compared to the leading competitor and are ideal for development of covalent gold nanoparticle conjugates.
The carboxyl functionalized gold surface allows for covalent conjugation of antibodies, proteins and other ligands using standard EDC/NHS coupling chemistry.
These gold nanoparticles have a narrow size distribution (CV of less than 15%) and more than 95% spherical particles.
Low batch to batch variability (+/- 2nm) assures you as a customer always end up with a product within the specified size range ordered.
All of our batches go through stringent quality control prior to shipment to our customers using dynamic light scattering to guarantee size and monodispersity and lot specifications are supplied with each product.
Storage
Store product away from direct sunlight at 4°C. Do NOT freeze. When stored as specified the product is stable for at least twelve months.
