Product overview:
Human IgG ELISA kit is a sensitive and accurate assay for the quantitative determination of human IgG levels produced in vivo and for assessing the level of immunoglobulin secretion by a hybridoma in vitro.
The Human IgG ELISA kit is based on the antibody sandwich principle. A microtiter plate coated with a capture antibody specific to human IgG Fc has been blocked and stabilized to create the solid phase of the assay. To perform the assay, samples, standards, and controls are added directly to the wells of the plate. After washing away unbound IgG, an HRP-conjugated Detection Antibody Solution is added and binds to the heavy and light chains of the captured human IgG protein that were immobilized by the capture antibody, completing the sandwich. The wells are washed and a tetramethylbenzidine (TMB) Substrate Solution is added. A blue colour develops in proportion to the amount of bound human IgG. The color development is stopped using Stop Solution, which turns the blue end-product to yellow and the optical density (OD) of the yellow product is measured at 450 nm on a microtiter plate reader.
Our ELISA kit has several benefits:
- Ready to use. Simply add standards and samples directly to wells. No need for lengthy blocking or rehydration steps.
- High sensitivity, specificity and reproducibility.
- Fully validated in the sample types listed below.
- 96-well plate is breakable into 12 x 8 well strips.
Protein name | Human IgG |
|---|---|
Species reactivity | Human |
Assay format | Solid-phase Sandwich ELISA (quantitative) |
Sample type | Serum, Plasma, Cell culture supernatant |
Sample volume | 100 μL |
Assay length | 3.5 hrs |
Analytical sensitivity | <50 pg/mL |
Assay range | 62.5 – 4000 pg/mL |
Intra-assay CV% | <6% |
Inter-assay CV% | <12% |
Recovery% | 98.7% (Serum) 96.2% (Culture media) |
Detection & Instrument | Colorimetric, Microplate Reader |
